Part:BBa_K4757064:Design

Ps1/Ps2-XylS/Pm-RBS1-SPpstu-FAST-PETase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1110
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 388
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 925
  

Design Notes

XylS and the Pm expression cassette is on different parts of the plasmid

Source

Sequence obtained by synthesis. XylS/Pm was on the pSEVA438 plasmid